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3rd Generation ELISA

HIV Infection

Hi There,

I have been to a lady a month back. I am little worried of myself now. What I did is I rubbed my penis on her vagina and while rubbing I inserted the tip of my penis and suddenly i realized and took off. Wore condom that had sex. But m still worried if I can get HIV transmitted. Since she was an unknown lady i am not sure of her. status. Please advice me. Thanks


I had a blood test done for hiv after 5 weeks but still haven't heard anything what kind of test would this be and as thet say no news is good news its been 9 days the results should be back by then in Canada yes what do you think

Positive Rapid Test but Negative ELISA test

I am a 32 year old male. I had an unprotected vaginal sex in mid December 2015. On January 26th (roughly 5 weeks after the exposure), I started feeling fatigued and had flush face for no apparent reason. Two days later, I had swollen lymph nodes (only in the neck), sore throat and oral thrush followed by a 38.2 degree fever.

The doctor told me it was probably tonsillitis and a throat infection that has caused the fever. I was given penicillin injections ( 3 applications, 1 daily for 3 days, 800 mg each) along with Paracetamol (500 mg capsules, 2 times a day) and Anti-inflammatory medicines (600 mg capsules, 2 times a day) for 3 days. My fever was gone in 3 days but I still had mild swelling in the lymph nodes with some pain and discomfort. The doctor did not feel necessary for me to continue with the anti inflammatory drugs and told me that the swelling will heal naturally.

On February 4th 2016, I told the doctor about my exposure to HIV risk and he said that although he doesn't think that I have HIV but it was wise to do a Rapid HIV test. The test was called "DoubleCheckGold" HIV 1&2. I got the result in approx. 1 hour and it was POSITIVE.
However, they said that they need to do a confirmatory ELISA 3rd Generation test to confirm. The ELISA test came out NEGATIVE. They ruled out the earlier test as a "False Positive".
I was asked to take another test 3 months after the exposure to be sure.

2 weeks later, I started having irritable bowel and diarrhea. The doctor gave me Omeprazol and IBS related medicines because I had a history of gastritis. However, they did not help. I saw a Gastroenterologist and he did not find anything serious and told me that had Irritable Bowel Syndrome. The doctor also said it was possibly due to stress and anxiety because of my HIV tests or anti inflammatory drugs. I still had swollen lymph nodes but no sore throat or pain. The stomach symptoms improved with medications.

I took ELISA 4th Generation Duo Test on March 30th i.e. roughly 3 months and 10 days after the exposure and it also came out NEGATIVE. 2 days earlier, the girl I had sex with also showed me her Rapid Test (which she took on March 25th) results and they were negative. However, I still have slightly enlarged lymph nodes although the doctor says he cannot see any swelling/redness but it definitely feels slightly bigger than normal. I also get flush face especially while eating. My blood test also showed that the percentage of lymphocytes was 15% and the normal range is between 20%-40%. According to the doctor, it was not something to be concerned about.

At this point, should I consider this test as conclusive or should I take another test in 3 months to be sure?

how conclusive is a negative result

what kind of checks are done to see that the accuracy of the rapid and EIA lab tests remain at about 99.6% sensitivity for true negatives? Outside the window period. And for how many people has this sensitivity ever been checked, how often?

Of course the more regularly one tests, the more certain one is of one's negative status, just by probability. But 99.6% on one test from the last encounter is still a chance of 4/1000 false negatives right? Or not?

No diagnostic screening test is clinically always 100% I have studied, so what is meant by the term "conclusive" after the window period.

Scared of hiv after unprotected oral sex and developed a pain under the armpit

I had an unprotected oral sex on 12 February and a week later I had developed a dry cough , fatigue and muscle stiffness with no fever and two days ago I had developed a pain under my armpit with no lumps and swelling and a pain on my chest muscle , before oral sex I had a bleed from my gum due to wearing of my braces while brushing my teeth in the morning and I had an oral sex at night and I had swallowed the cum since I had heard that the stomach enzyme would kill all the virus. Do you think I migh get hiv infection . I have not when for an his test since since it is not three month,

About the CMIA test

Hello! I got a CMIA test at day 83 after a potential HIV exposure via unprotected vaginal sex. I got a negative result (0.28 result, cut-off value is 1), and I believe it is conclusive. However, I have a question about the CMIA test. Is the CMIA a 4th gen test, or are there older 3rd gen CMIA tests still used? If all CMIAs are indeed 4th gen tests, I would go for them over other standard testing formats for routine HIV checks. Please advise.

Thank you!

high risk and test result

first sorry for my English, i have been here for the last 6 months reading posts and giving me courage to wait for testing. after all this tie i have some question if someone can answer them please.
long story short by curiosity i met this trans over internet. we had sex i was top with condom and then i was bottom while i was bottom the condom slipped but she continued without stooping. after that i get freaked out i almost lost my mind i had some "symptoms" and then

4 weeks 3gen HIV1/HIV2 > negative
7 weeks 3gen HIV1/HIV2 > negative
12 weeks 4gen ab/Ag > negative
4 months 4gen ab/Ag > negative
at 6 months full std panned > all come back negative
now 6 months and 2 week 4 gen ab/Ag > negative

is test result always overrule exposure risk ?

1/what scares me the most is i m going to get married in 3 weeks, can i consider myself clear and no more testing ?

i lived in hell for the past 6 months tried every anti depressant/anxiety dug just to support the waiting

thank you in advance


I need your help I am to much in stress … I took my HIV 1-2 ECLIA TEST on 77 days .. its came result 0.32 (Non-Reactive).
I had only 1-2 min Virginia Sex with sex worker . I was too much under pressure boz doing first time outside girl. Only one time I did and lot of doubts I am facing.

Kindly let me know is it final result. Or need some more test ?

I had symptoms since first day not sure its by Stess lot I was carrying. Now I have Swelling also in my full Hand, Leg with Finger & EyeLid, Leg and Hand I feel low energy.

Kindly advise me what should do now …? plz reply asap

Thanks & Regards,

Inconclusive Results, ARS, Braces


I recently was tested for HIV and when my results came in I was called into the testing clinic. These were the results:

HIV 1/2 Ag/Ab Combo Screen: Reactive
HIV 1 p24 Antigen Screen: Non-reactive
HIV 1 Western Blot: Negative
HIV Final Interpretation: No HIV p24 antigen detected; inconclusive for HIV1/HIV2 antibodies

I have had two sexual partners in my life. With one we only had oral sex. With the second more recent one we had sex twice and used a condom both times (10 weeks prior to the first test) and the person that I had sex with said that he had been tested and was HIV free prior to sex. I am gay and was bottoming. Based on my history I questioned the result and immediately went for point of care rapid testing. The test came back negative and the doctor said that he questioned the initial result, thinking that there may be a non-HIV antibody in my blood triggering a false positive. However, he told me to abstain and sent off my blood for additional testing. I have about a week to go before I hear back. I have a few questions:

1. I have braces and I kissed someone deeply three weeks before the first test. Could I have contracted HIV in this way?
2. I have had cold/flu symptoms and swollen glands on and off for the past 3 or 4 weeks. Could these be symptoms of ARS? The symptoms seemed to be resolving and then worsened with the stress from the news of the first test.
3. What is the HIV 1/2 Ag/Ab Combo Screen? Is this the same as ELISA? How does it differ from the other tests that came out negative? What could lead to a false positive on this test?

Any info that you could provide me with would be great. Thanks.

How Scared should I be?

Hello- I had sex with a stranger In January of this year. I wore a condom the entire time- and it did not fail (ie break). I am a male and it was with a female. I took a Oraquick at home test 8 weeks post exposure with the results being negative. Is this enough information to begin moving on with my life?


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