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3rd Generation ELISA

HIV tests at 90 days - Still having fear

Hi All,


I am from india, I had a brief exposure with a prostitute in honkgong exactly before 99 days. I used condom, as this is the first time I was having vaginal sex, but I don't was there any break or slippage.

I was licking her vagina for 10 seconds and kissing her continuously in nipples, mouth.

I started to get lot of symptoms from 20 th day onwards

Rashes, mild fever, 3 small lymph nodes in neck, weight loss of about 5 kg, sometimes high fever about 100 f.

I started my tests as follows,

29the day rapid test - negative
33rd day rapid test - negative
33rd day REAL TIME pcr - viral load - hiv 1 - Target not detected
43rd day - elisa - non reactive
49th day - HIV DUO CMIA - non reactive
56th day - elisa - negative
63rd day - elisa - negative
70th day - elisa - negative
77th day - HIV DUO CMIA - non reactive
81st day HIV 1 RNA DETECTION using cobas taqman - not detectable
90th day - HIV DUO CMIA - non reactive
90th day - ELISA - negative

Other than these tests, I took STD tests on 88th day - for chlamydia, syphilis, HSV 1 and 2, HbsAg, CMV, RPR/VDRL, TPHL, TOXOPLASMA, rubella. Everything is negative other than rubella IgG with value of 136.

1. Is my tests are conclusive, should I need to take any tests in 6 months,

2.from 2 week onwards I am getting rashes on/off. is it related to HIV

3. What could be the cause of lymph nodes near ear and neck, this are not noticeable and not paint, May be around 1 cm.

4.I was using Alprazolam 0.25 MG for anxiety, will this have any effects on HIV tests.

5.is my other STD tests are conclusive?

worries about HIV

Hai Sir,

I am having as unprotected sex 20 years back.I check after 3 years (Elisa test) its comes negative , And now last 10 years iam working in dubai.Every 2 years thay are taking blood for for testing (i hope so) & all are passed.

Now the problem i feel is a lymph node on right side of my neck (i took heavy antibiotic but not going down) & feel too much tired.

Is this any chance of HIV. Iam too much worried,Please help me sir.



I've been through two incidents prior to this, the first (unprotected sex) and second (dabbing) which had caused me to live in anxiety for a long time. However I was tested negative for both situations (multiple tests over three months period after exposure) and stopped having sex.

However recently I just got into another incident, where the guy did not have a condom and rubbed his penis on me for minute? I immediately told him to stop after feeling a slight jab on my anus. I don't think it penetrated, there was no lube and I did not feel any pain at all. I was also able to stop the movement with my buttcheeks. I fear it penetrated, since I did not have sex for a long time and rarely do in the first place I would not know. Also there was no fluid, as the whole situation was very abrupt.

Again now im suffering from anxiety and counting the days until i can get tested in six weeks, meanwhile unable to do anything because of my agonizing mind. It may be because I had such bad prior experiences, that im so used to worrying. I was hoping that you could briefly asses my risk? also for future reference, what signals/feelings constitutes that something has penetrated into me?

HIV test

I am Prince from UAE. I recently did an HIV test using ECLIA. The result for me was negative. Now the recommended range showin the test is above 1 is positive. The value recorded for me was 0.2. My question is,

Is it common to have small traces of HIV virus in humans? Can this be cured completely?

Also is Hepatitis B transmitted by sex or is it by any other means? My Hepatitis B test was also done using ECLIA and my recorded value was 0.53.

Can this also be reduced?

HIV transmission

i had sex with three guys but note that i had done it at different time . but i have done it without condom is there any risk of HIV

pcr dna and p24combo test

One of my friend participated in sex with a girl but the condom was broken in middle...he completed his sex after that he find it was broken...any way he tested with pcr dna qualitative test after 22 days from exposure the result is negative...and after 35 days from exposure tested with hiv duo p24 combo test .
the result is negative. After 60 days from exposure tested with same p24 combo test result is negative...pls anybody can give detailed information about this early tests...we saw in some websites the pcr dna test works 2 to 3 weeks...after 3 weeks or after a month or later the pcr dna test detect the virus or not ...it detect only in early stage like 2 to 3 weeks...or the pcr dna qualitative test detect virus after 3 months or not ..and also the antigen p24and hiv duo combo test also mentioned that after the seroconversion period the antigens are undetectable...
We are in confusion..the p24 combo test results was valid or not after 5 weeks and 8 weeks after exposure...
if test with pcr rna qualitative test is that usefull after 2 months from exposure
my friend dont have any symptoms during this 2 months...but he had sex with his wife and he used condom during the sex..but he kissed her with lips with sour lips..both of them have sour lips cause of summer heat..
is there any problem with lip sour contact with kissing...pls answer with full details...thanq u.

HIV risk


A bit over six weeks ago I had an unprotected encounter (condom broke) with an HIV+ guy, I was the receptive partner (anal sex) . I found out about his status too late to start prep, therefore, I know there might be a high chance of infection. I have had a variety of symptoms since then, and was also tested positive for Chlamydia, I have had cold like symptoms (Sneezing, runny nose, swollen glands)- no fever, nor diarrhea, nor vomiting tho. I have been dealing with a nasty headache on a daily basis almost since day 4 after the incident, however , I have tested negative at 3 weeks (rapid test), 4 week ( P24 antigen) and 5.5 weeks ( 3rd generation Elisa), and although I know these tests aren't conclusive, is my negative 5.5 weeks somehow reassuring ? I am freaking out, and the headache, combined with the stomach pain I have developed after a course of antibiotics to treat chlamydia are taking a toll on my sanity and peace of mind, so thank you for your input!

HIV Infection

Hi There,

I have been to a lady a month back. I am little worried of myself now. What I did is I rubbed my penis on her vagina and while rubbing I inserted the tip of my penis and suddenly i realized and took off. Wore condom that had sex. But m still worried if I can get HIV transmitted. Since she was an unknown lady i am not sure of her. status. Please advice me. Thanks


I had a blood test done for hiv after 5 weeks but still haven't heard anything what kind of test would this be and as thet say no news is good news its been 9 days the results should be back by then in Canada yes what do you think

Positive Rapid Test but Negative ELISA test

I am a 32 year old male. I had an unprotected vaginal sex in mid December 2015. On January 26th (roughly 5 weeks after the exposure), I started feeling fatigued and had flush face for no apparent reason. Two days later, I had swollen lymph nodes (only in the neck), sore throat and oral thrush followed by a 38.2 degree fever.

The doctor told me it was probably tonsillitis and a throat infection that has caused the fever. I was given penicillin injections ( 3 applications, 1 daily for 3 days, 800 mg each) along with Paracetamol (500 mg capsules, 2 times a day) and Anti-inflammatory medicines (600 mg capsules, 2 times a day) for 3 days. My fever was gone in 3 days but I still had mild swelling in the lymph nodes with some pain and discomfort. The doctor did not feel necessary for me to continue with the anti inflammatory drugs and told me that the swelling will heal naturally.

On February 4th 2016, I told the doctor about my exposure to HIV risk and he said that although he doesn't think that I have HIV but it was wise to do a Rapid HIV test. The test was called "DoubleCheckGold" HIV 1&2. I got the result in approx. 1 hour and it was POSITIVE.
However, they said that they need to do a confirmatory ELISA 3rd Generation test to confirm. The ELISA test came out NEGATIVE. They ruled out the earlier test as a "False Positive".
I was asked to take another test 3 months after the exposure to be sure.

2 weeks later, I started having irritable bowel and diarrhea. The doctor gave me Omeprazol and IBS related medicines because I had a history of gastritis. However, they did not help. I saw a Gastroenterologist and he did not find anything serious and told me that had Irritable Bowel Syndrome. The doctor also said it was possibly due to stress and anxiety because of my HIV tests or anti inflammatory drugs. I still had swollen lymph nodes but no sore throat or pain. The stomach symptoms improved with medications.

I took ELISA 4th Generation Duo Test on March 30th i.e. roughly 3 months and 10 days after the exposure and it also came out NEGATIVE. 2 days earlier, the girl I had sex with also showed me her Rapid Test (which she took on March 25th) results and they were negative. However, I still have slightly enlarged lymph nodes although the doctor says he cannot see any swelling/redness but it definitely feels slightly bigger than normal. I also get flush face especially while eating. My blood test also showed that the percentage of lymphocytes was 15% and the normal range is between 20%-40%. According to the doctor, it was not something to be concerned about.

At this point, should I consider this test as conclusive or should I take another test in 3 months to be sure?


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