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Test Accuracy

Hi, i had protected sex about 8 weeks ago with commercial sex worker. I did fingering in to hers vagina and post that had vaginal sex. I had done DNA PCR test on 15th day was negative. I have done Antibody Elissa test on 57th day which is post 8 weeks of possible intercourse was again Negative.
Can I consider my test results conclusive?

Multiple negative test conlusive?

Hello, I had 1 protected enclosure about 16 weeks ago, I tested PCR RNA negative at 2 weeks, then negative 4gen combo (antigen and antibody) at 28 days and again negative at 13 weeks. Then I tested negative rapid blod test at 14 weeks. Are these negative results conclusive?

Is there any change I can get positive after? Do I need to test at 6 months? Can antibodies be detected at a further point or with just the antigen and RNA is fine to rule out a a possbility of infection? I want to start my monogany sex life again with confidence!

rna qualitative pcr

hi if rna test accuracy is 99.6 after window period and if result is negative has there been any cases of false negative after window period . is there any possibility by which rna test cannot find virus after 21 day window period

Queries ? Repost ?

Last test was take at 179th day ! Hiv1/2 ag/ab ( Eclia ) Non reactive!

Got tested at 23rd 28th 42nd 93rd 122nd 179th day !
All were hiv 4th generation tests !
One PCR rna test ( limit was 66.6ml copies ) All came out negative!
One NAT test ( viral screening hiv1/2 hep b hep c ( Roche Cobas taqman )

1) are this conclusive ?

2) any further tests needed ? At 9months or 12months ?
3) do I need to test by western blot ?
4) any possible of all tests being false negative ? 5) symptoms ? Improper stools ? Minor sore throat ? Bad feeling about being infected !
6) diff between NAT and id NAT tests?

? Queries ? Confused

PRespost Last test was take at 179th day ! Hiv1/2 ag/ab ( Eclia ) Non reactive!
Got tested at 23rd 28th 42nd 93rd 122nd 179th day !
All were hiv 4th generation tests !
One PCR rna test ( limit was 66.6ml copies ) All came out negative! One id NAT test - viral screening ( Roche Cobras taqman ) hiv1/2 hep b Hep c

1) are this conclusive ?

2) any further tests needed ? At 9months or 12months ?
3) do I need to test by western blot ?
4) any possible of all tests being false negative ?
5) symptoms ? Improper stools ? Minor sore throat ? Bad feeling about being infected !

Test Results Question

I recently had a sexual encounter with a transsexual female (born male). I received unprotected oral sex and we had protected anal sex (condom). She ejaculated, cleaned off and then gave me oral sex plus anal fingering. I have no idea how well she cleaned up after ejaculation and if there was any left on her body/hand which was then used back on me in form of handjob and anal fingering. I also had slight open abrasions on my penis from previous sex that week which had not fully healed.

The day after I had shooting migraines that lasted 2 weeks. At 14 days after possible exposure my lymph nodes swelled and became sensitive to touch. I also woke up on day 14 in a panic/sweat. I had blurred vision and was experiencing dizzy spells with waves of nausea.

We both got tested the next week (9 days, 21 hours after the sexual encounter). Not sure what test she got but I believe a full test panel (but the HIV test must have been rapid because she got results right away). Results on that test were negative for everything.

My test (same exact time frame, 9 days 21 hrs) was performed using the APTIMA HIV-RNA Qualitative Assay (Gen-Probe). Test came back negative “HIV 1 RNA, QL TMA NOT DETECTED, REFERENCE RANGE: NOT DETECTED”

My question is am I definitively clear of possible HIV exposure or do I need extra testing? Is it possible that the test I got was done too early? I've since read that 11 days post-exposure are needed for that test to be definitive.

I ended up in an urgent care unit today due to the amount of anxiety and stress I am feeling about this entire situation. My heart felt like it was going to burst through my chest I am so nervous. Can't focus and am feeling depressed and overwhelmed. I feel a slight bit of relief with this negative test result but I am not convinced…not sure I will be convinced for a long time the way I am feeling…

Thank you for your response and help

Hiv? Re post ? Final queries?

I had a question posted but haven't got any reply !

But let me describe again.

Hello doc !
I did have a possible exposure to local sex worker in Greece ( MAY 2015 )

Symptoms did arrive post 2-3 weeks
Exact flu kinda symptoms!
Was nervous and thought of being infected!
Really felt like killing myself !

Some sources say 3 months some say 4weeks some 6 weeks some 6 months too !

Some say there are 4 situation which effects the hiv testing !
1) pep
2) treatment for hep c
3) treatment for cancer
4) immunodeficiency

How do I know whether I have immunodeficiency ? I was serious kinda ill during my childhood
Suppose if I do I have some immunodeficiency
Does it affect my testing !?
Testing is delayed for antibodies ?

But even with some immunodeficiency antigens /rna/ dna / Id NAT should be detected?it detects the genetic material of hiv

I have take several duo test at
23rd day - Eclia
28th day - Eclia
42nd day - Eclia
58th day - Eclia
89th day - ELFA
93rd day - Eclia
103rd day - ELFA
103rd day - Cmia
122nd day - cmia

And last one was

179th day - Eclia ! All were not reactive


One hiv rna pcr 1 qualitative @ 103rd day - NOT DETECTED

One id NAT test @ 125th day - viral screening for hiv rna Dna 1/2 ! Hep c ! Hep b ! - NOT DETECTED

Two complete immunology test once th day when I had my IgM was slightly high and IgG was slightly low @ 48th day

Again took immunology test at 58th day
All were normal

Again took immunology test at 93rd day
All were normal

Agin took immunology test at 179 th day !
All were normal

Took syphills test at 23rd 42nd 58th 93rd
And 179th day -all non reactive

Took herpes 1/2 IgM IgG non reactive at 42nd day
@ 93rd day IgM coming out as equivocal and IgG was non reactive
@103/104 ( two different lab ) - both IgG IgM was no. Reactive

Took hepatitis b antigen @ 58th and 93rd day non reactive and 179th day - non reactive

Took chlyamdia IgA IgM IgG -negative at 42nd day

Anti HCV - 93rd day and 179th day -not detected

60th day - CBC count was normal
89th day - CBC - lymphocytes full high - neutrophils low
93rd day - Cbc - lymphocytes slightly high rest normal
103rd - Cbc - lymphocytes slightly high rest normal
124th day - lymphocytes slightly high

130 th day - all were normal

179th day - lymphocytes are high rest all are normal

Took vitamin d and vitamin b 12 - Have Deficiency ( I am vegetarian)

Really tensed !

Got some symptoms that is
Sometimes improper stools or dark colored stool !
Minor sore throat !

Still have bad dreams ! Being too anxious !

Can you explain ? This in detailed manner ?

So many duo test ? Yet feeling scared !

I got married recently! I don't wanna infect her !

I got weird throat sometimes it's proper sometimes it's not same with my stools !

Can all this tests be false negative? That hiv PCR rna ? Duo ? Id NAT for hep c hep b hiv1/2

What is the difference between NAT and id NAT test?

I had three immunology test once Igm was high and IgG was low ! Rest immunology tests were normal !

But lymphocytes are high ! Mostly since two months - three months ?
Any testing needed ?

equivocal eia and subsequent rna naat

Feeling very anxious and looking for some guidance please.
My boyfriend went for routine bloodwork 6 weeks ago incl an hiv test. His dr said his first test was inconclusive but the second test was fine so he has nothing to worry about. I asked my boyfriend what the deal was with his first test - he doesnt know. He showed me the test results - "eia equivocal. Ag (screen) very abnormal" . The western blot was not done as a secondary bc "low eia signals" dont allow for useful results. So an rna naat was done - no rna found - "these tesults are inconsistent with hiv infection" . His drs final conclusion was " nonreactive"
I'm wondering:
1. What does the very abnormal on the Ag screen mean?
2. Why wasn't a western blot done?
3. How conclusive is the overall result since the rna naat came back clean?
I am so overcome with anxiety I cant take it another day. Ive spent sbout 40 hours online trying to sort this out and it has just made me more anxious. I just need an interpretation bc trying to do it myself is killing me. Please help me.
Thank you

hiv duo at 40 days

Dear Sir/Madam,
I have done a stupidest thing,I had 4 sexual encounters with 4 different sex workers and each sexual encounter was at a gap of approximately 1 week and all sexual encounters was done using condom. My last sexual exposure was on 25th September. From 26th onwards i have different symptoms like a rash on chest ,fatigue, ill like feeling and itchy rashes on my penis and anus,white thrush on backside of my tongue .After 18 days of my last sexual encounter I had RNA PCR qualitative test and the result came as "not detected" later on day 40 after of my last sexual encounter I had 4th generation HIV DUO test which came as non reactive and index value is 0.16.I heard that hiv duo test at 6 weeks is conclusive,is my HIV DUO test is conclusive?or should i have to test again? please reply and help me, thanks in advance.

Risk of breast licking

Hi Team,
The only exposure i had on 28th of august is that i licked the breast of a CSW for 2 minute(softly). There was no intercourse happened(vagainal or Anal). I have done HIV NAAT test right after 30 day of exposure it came as negative.
Symptom i faced are below:
White toungue and dry mouth (after 9 days)
Diarrhoea (2 week after exposure)
Burning sensation( 2 week after exposure)
A fever (101) and swollen lymph node (after 60 days of exposure).

Please help i am really worried.
My 90 day are going to complete on 28th of november.
Do i need to go for testing? Really worried.


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