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Pooled RNA/Early Test

Testing anxiety

Hi there, I took a rapid finger prick test at 33 days (1 month) post possible exposure.
It was negative. I was a low risk scenario and had caught a cold 2 weeks after the possible exposure, my underarm turned red for a few days and a light reddish area near the side of my back...but this was all before the test and the nurse said if I was symptomatic of HIV the test would of picked it up. I'm near 7 weeks now and fighting myself to get another test? Should i? Would that 33 day negative change? I'm not a drug user or have been on cancer treatment....please help me, thank you!

Article that doesn't make sense

I am reading this article on Mayoclinic website and it doesn't make much sense.
I am pasting couple of disturbing lines here.

Antibodies against HIV-1 and HIV-2 are usually not detectable until 6 to 12 weeks following exposure and are almost always detectable by 12 months. They may fall to undetectable levels (ie, seroreversion) in the terminal stage of AIDS when the patient's immune system is severely depressed.

----- Why it says it can take up to 12 months to be show the virus ------ when it takes 3 to 6 months ?

Negative results for both HIV-1 and HIV-2 antibodies usually indicate absence of HIV-1 and HIV-2 infection. However, in patients tested reactive by a fourth generation HIV antigen and antibody combination immunoassays, such negative results do not rule out acute HIV infection. If acute HIV-1 infection is suspected, detection of HIV-1 RNA (HIVQU / HIV-1 RNA Quantification, Plasma) or HIV proviral DNA (PHIV / HIV-1 Proviral DNA Qualitative Detection by PCR, Blood) is recommended.

Negative results for both HIV-1 and HIV-2 antibodies do not rule out acute HIV infection. If acute HIV-1 infection is suspected, detection of HIV-1 RNA (HIVQU / HIV-1 RNA Quantification, Plasma) or HIV proviral DNA (PHIV / HIV-1 Proviral DNA Qualitative Detection by PCR, Blood) is recommended. For patients at risk for HIV-2 infection (eg, having lived in West Africa or have sexual partners from West Africa), testing for HIV-2 DNA / RNA (FHV2Q / HIV-2 DNA/RNA Qualitative Real-Time PCR) is recommended.

----- what are they trying to say that when they say negative test doesn't rule out the acute infection ---

Here is the link of that article:

Low risk activity with a positive test result. How??


In April 2015, my husband and I had a threesome with a male acquaintance. I did not know his status at the time. I engaged in unprotected oral (giving and receiving) and protected vaginal with condoms and the male not ejaculate. My husband did not engage in any activity with the male. Just me.

In May we did the same with another male though there was no vaginal sex, I did give and receive unprotected oral without ejaculation. My husband did not engage with the male, just with me.

On June 21, we had another threesome with a third male acquaintance of unknown status. I engaged in unprotected oral and vaginal penetration without ejaculation on the part of the male. I did use my hand to bring the male to ejaculation (masturbation).

In July my husband and I travelled to Bangkok and received body to body massages by two women. There was no oral, vaginal or anal sex. We bathed together and touched each other only. They kissed my neck, ears and back as well as manually stimulate my husband and myself.
On a separate occasion in Bangkok my husband received 2 massages ending in a hand job and the masseuse fingered his anus without protection.
Two weeks after this, my husband came down with flu-like symptoms. We both got tested for HIV using Aptima HIV-1 RNA from a nearby lab. My results were negative and his were positive.

We are getting retested and getting his viral load and CD4 now.

I thought we were only engaging in low-risk or negligible-risk activities. Is there any chance he got a false positive? Prior to April of this year, we have been monogamous for ten years together. And we do not use drugs. No blood transfusions. I don't understand how this could happen?? I've read all the numbers calculating the risk of each activity and it seems more likely that he got a false positive. Looking for advice or thoughts. Our doctors don't seem to believe us or care how it happened.
Please help.

Unprotected and tested

Hi, I live in the United States and I had unprotected sex with a female and she has said that she is safe from all diseases and that she has been tested before. I was still concerned because I had a small rash on the head of my penis a few days later and went to my urologist and told me it was fungal and cream cleared it up.

I still had following test done to make sure I
33 days after exposure HIV 3rd generation test negative.

49 days after exposure HIV 4th generation combo test and HIV RNA test (that detects the virus directly and conclusive at 28 days) both negative.

77 days after exposure HIV 3rd generation combo test was also negative

84 days after exposure HIV 4th generation test negative

126 days after exposure HIV 3rd generation test along with STD panel for other STDs also all negative.

I was told that 84 days is conclusive and no other testing is needed. I have thyroid condition caused by and autoimmune issue but was told that doesn't fall under the 4 categories that would delay an HIV test. Could you please help and tell me if my result are indeed conclusive. For some reason there are no websites in the USA that compares to your website with all the information you have. I would appreciate any help and thank you.

Brief receptive anal penetration

I am gay in my 30s and had sex with another guy. I was the bottom and we used a condom. In the middle of sex, I told him I'm in pain and to stop. He proceeded to take off the condom and then out of nowhere he suddenly pushed his penis inside me. I immediately pushed him out. I would say his unprotected penis was inside me about a quarter of the way in for about 2-3 seconds. He had pre-cum. The following day, I asked him if he was healthy, and he said yes (no proof of course). He didn't even bother asking me my status. I'm really worried. Do you know people who tested positive from such cases where there was brief exposure?

Risk of exposure

On the 26th of June, I had protected sex with a woman younger than me. While we were having sex I noticed a very unpleasant odor, so much that I had to stop. this odor made me extremely worried.

I went through an Internet STD check service where I had a full 10 panel STD check to include the 4th Gen test and an RNA quick detection test, all blood drawn.

The tests were 2 weeks and again 4 weeks after possible exposure. Both tests came back negative.

Should I still be worried?

HIV risk and test period.

hello, at first sorry for my bad English and thank you very much for your replies and interest

5 days ago, I recently engaged in protected oral sex with a woman of unknown status. I did not ejaculate during the oral. The woman wear the condom to my my penis with her mouth. during the oral sex, i touch her vagina for a very short time and fell a litte wet vaginal fluid. İ did not put my finger inside her vagina, only i touch her vagina nearly 5-10 seconds...

After the protected oral sex , i took the condom off with my hand and then she began to give me a handjob with baby massage oil. After about 2 minutes later i touched my penis too and masturbate. then i cum.. before and after the protected oral sex i kiss her lips for a short time.. it was very a soft kiss.

1 ) Is it likely that if her vaginal fluid was infected and touched my penis head, then the force of masturbate would force the little bit of fluid down my urethra? I had no cuts or sores on my penis or scrotum. I am very worried . after this event, nearly 2-3 hours later i had a sore throat. every time i worry about hiv risk, i have sore throat .

2 ) wearing condom to my penis with her mouth is a risk? ( because of salva vs )

3) the time between touching her vagina and then touching my penis is nearly 2 minutes.. and i have no cuts or sores in my finger.. the vaginal fluid is so little and if there was virus in my hang ,could it be decay in two minutes because ot air contact?

4) i read lots of articles about test period. after 9/10 days later PCR RNA would give an idea about my situation. and 14th day vidas duo test ?

please help me..
thank you again

can HIV transmitted from breast secretion?

Hi every one
Before 8 weaks I been with working girl and I sucked her nipple for few minutes I'm not sure if she spread any thing to my mouth or not ( I did not test anything) after 4 weaks I take the RNA qoulitive test and come back negative
The questions is
1-Can hiv transmitted through breast secrection ?
2-is this result consider convulsive? If not wich test you recommend me to take and when
3- if she spry anything to me is that testable?
Thank you so much

hiv risk in anal sex and window period for tests


I had anal sex with a ladyboy in pattaya around 20 days back and my condom broke. I cummed early, the duration lasts for around 10 seconds only and after I cummed I saw that the condome was broken and the front part of the condom was inside him, he took that out and showed me that I have cummed inside the condom only, but still I am very much tensed. He told me that he is not infected, but I cannot trust him as i dont knnow his status. I am not experiencing any health issues as of now.

today I went to a clinic and they asked me to undergo some tests which include cbc c gsr, rft/lft , bld sugar.R, hbsag/hiv , urine -R, widal and all the reports comes normal so he said that there is no need to worry, there is very low chance that you have hiv, but I am still very much tensed, so he asked me to go for viral load tests,western blot for hiv, hiv Eliza and hiv viral load. So I want to know the window period for these tests and if these tests comes negative then what is the next step, can these tests will gve me some relief ??


Hi sir.My question is ....is there difference in window period according to type of risk?One person said me that when very high risk exposure like blood transfusion of hiv positive person to hiv negative person occurs then body produce detectable antibody in short time because high amount of virus injected during this type of exposure and when exposure like unprotected sex occurs then body takes long time to produce detectable antibody because small amount of virus enters in body by this type of exposure.IS THIS TRUE??Now i am telling my exposure before 7 months i had done sex with csw by wearing condom during sex my condom has broken and immediately i pull up my pennis and ejecuted outside her pussy.i noticed that my pennis was dry and no body flood on it.After this type of very low risk exposure after 7 months i have done hiv duo test which is negative. Is my test conclusive for both hiv1 and hiv 2 because no antigen for hiv type 2 ?.Is this enough time to get conclisive result after this type of very low risk exposure ?I have read in your posts that most people develope detectable antibody in 21 TO 25 days. Is this figure related to very high exposure like blood transfusion?


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