« Go Back

Pooled RNA/Early Test

HIV risk and test period.

hello, at first sorry for my bad English and thank you very much for your replies and interest

5 days ago, I recently engaged in protected oral sex with a woman of unknown status. I did not ejaculate during the oral. The woman wear the condom to my my penis with her mouth. during the oral sex, i touch her vagina for a very short time and fell a litte wet vaginal fluid. İ did not put my finger inside her vagina, only i touch her vagina nearly 5-10 seconds...

After the protected oral sex , i took the condom off with my hand and then she began to give me a handjob with baby massage oil. After about 2 minutes later i touched my penis too and masturbate. then i cum.. before and after the protected oral sex i kiss her lips for a short time.. it was very a soft kiss.

1 ) Is it likely that if her vaginal fluid was infected and touched my penis head, then the force of masturbate would force the little bit of fluid down my urethra? I had no cuts or sores on my penis or scrotum. I am very worried . after this event, nearly 2-3 hours later i had a sore throat. every time i worry about hiv risk, i have sore throat .

2 ) wearing condom to my penis with her mouth is a risk? ( because of salva vs )

3) the time between touching her vagina and then touching my penis is nearly 2 minutes.. and i have no cuts or sores in my finger.. the vaginal fluid is so little and if there was virus in my hang ,could it be decay in two minutes because ot air contact?

4) i read lots of articles about test period. after 9/10 days later PCR RNA would give an idea about my situation. and 14th day vidas duo test ?

please help me..
thank you again

can HIV transmitted from breast secretion?

Hi every one
Before 8 weaks I been with working girl and I sucked her nipple for few minutes I'm not sure if she spread any thing to my mouth or not ( I did not test anything) after 4 weaks I take the RNA qoulitive test and come back negative
The questions is
1-Can hiv transmitted through breast secrection ?
2-is this result consider convulsive? If not wich test you recommend me to take and when
3- if she spry anything to me is that testable?
Thank you so much

hiv risk in anal sex and window period for tests


I had anal sex with a ladyboy in pattaya around 20 days back and my condom broke. I cummed early, the duration lasts for around 10 seconds only and after I cummed I saw that the condome was broken and the front part of the condom was inside him, he took that out and showed me that I have cummed inside the condom only, but still I am very much tensed. He told me that he is not infected, but I cannot trust him as i dont knnow his status. I am not experiencing any health issues as of now.

today I went to a clinic and they asked me to undergo some tests which include cbc c gsr, rft/lft , bld sugar.R, hbsag/hiv , urine -R, widal and all the reports comes normal so he said that there is no need to worry, there is very low chance that you have hiv, but I am still very much tensed, so he asked me to go for viral load tests,western blot for hiv, hiv Eliza and hiv viral load. So I want to know the window period for these tests and if these tests comes negative then what is the next step, can these tests will gve me some relief ??


Hi sir.My question is ....is there difference in window period according to type of risk?One person said me that when very high risk exposure like blood transfusion of hiv positive person to hiv negative person occurs then body produce detectable antibody in short time because high amount of virus injected during this type of exposure and when exposure like unprotected sex occurs then body takes long time to produce detectable antibody because small amount of virus enters in body by this type of exposure.IS THIS TRUE??Now i am telling my exposure before 7 months i had done sex with csw by wearing condom during sex my condom has broken and immediately i pull up my pennis and ejecuted outside her pussy.i noticed that my pennis was dry and no body flood on it.After this type of very low risk exposure after 7 months i have done hiv duo test which is negative. Is my test conclusive for both hiv1 and hiv 2 because no antigen for hiv type 2 ?.Is this enough time to get conclisive result after this type of very low risk exposure ?I have read in your posts that most people develope detectable antibody in 21 TO 25 days. Is this figure related to very high exposure like blood transfusion?

When should one get tested

If you had a unprotected sex with anyone, after how long should you go for testing, so that test results are final & conclusive

Can my test results change in 5 months?

Is it possible for my negative four almost five month test to change.(test ordered was hiv ab 1/2 with reflex which was non reactive ) I just got tested for my tenth month test currently waiting on results. I have not had any other exposure. The last sexual exposure was in late September 2014. What are the chances of my test results changing? Also can you explain what "reflex" means on test.

false positive rapid test?

I am a gay male who went in for my regular semi-annual hiv test at a random free clinic. I was not testing over any particular exposure, it was just a check-up (i always use condoms for anal sex, but sometimes i perform oral sex with ejaculation in my mouth). They used a rapid finger prick test on me, and to my surprise the test came back "weakly reactive," with a very very faint grey line; additionally, the purple color of the top control line was bleeding down the test strip, in a big purple smear. These unusual factors, combined with my lack of high-risk exposures, made my counselor hesitant to call the test "positive." He retested me twice with 2 more rapid tests, both of which came back negative. At that point he, along with another counselor that he consulted with, were comfortable dismissing me with a negative result. On that same day, i went to another clinic where a fourth rapid test was performed (negative), and blood was drawn for a "pooled NAAT" test. The results of the NAAT came back negative as well. Prior to these testing events, the only "exposures" i could think of were instances of receptive oral sex with ejaculation that occurred one month and 2 weeks beforehand.

After that battery of tests, i waited one full month after the date of the "bad test," and returned to have 2 more rapid tests performed on me, along with full blood draws for a 4th generation elisa, as well as a second pooled NAAT test. All 4 tests came back negative. On paper, these tests were performed 6 weeks since any sex I had whatsoever.

Finally, after that, I waited 7 more weeks to hit the 3 month/13 week mark from any potentially infectious sex I had, and had one more rapid test, as well as a full 4th generation lab elisa, both of which came back negative.

Is this enough evidence that the first faint line test was falsely positive? I know very well that any test with a second line must be interpreted as reactive, no matter how faint the intensity of the line is, however I have had 10 subsequent negative tests taken over the course of 3 months, with the most recent test being performed 95 days since any sex i've had whatsoever, and a total of 81 days since the date of the first positive test. I guess i still feel uneasy because I never had a western blot, or any of the usual confirmatory tests used to rule out false positives. Can I rely on the test results i have now, especially since they are beyond the official 3 month window period?


Dear Sir / madam, I had protected sex with one of my female colleague. After that I never bothered of contacting to HIV. But after 18 months I started feeling mounth discomfort and and rashes on my tip of penis. Then I went to test for ELISA, WESTERN BLOT, AND PCR RNA. all three came back negative. But still I am not able to convenience myself that I am negative. Now I have started feeling pain in my groin area, armpit. Pls help me whether I am 100% HIV negative.

test pcr and 4th generation at 67 days is conclusive?

Im female i had sex with one men and he is positive hiv 1 was in treatment (i learned after about his status )..we used condom but as he tried to came inside the condom broken i heard the sound was out but i worry that seconds he was in contact with my vagina if i infected..i went to clinic they dont give me treatment cos i was not on risk..i had symptom like cough low fever till 37.2 the evening and pain in my arms and my tongue was white and i had tremor..i went and psychiatric and said all is from my stress..cos im very very bad..
36 days 4th gen antigen 24 negative
53 days 4th gen antigen 24 negative( and hep c -hep b -syphillis-herpes was negative)
67 days pcr rna and 4th gen antigen 24 negative
also i did vaggine for hep b
is conclusive my test?
does vaccine for hep b affect my test?
ALSO (and this is very important for me )my daugther used my toothbrush by mistake after 5 hours and she told me that wash it with her hands before dhe used nad there wasnt blood on it...im afraid if she infected from me if i am and the tests didnt saw that yet
im waiting your response with anxiety
thank u

thank u for your time

Tired of testing - what to do, please

Hi. Please help me.

Last year I took an HIV test at 12 weeks post last exposure (sex without protection with a woman that I dont know). This one came out as:

1) ICMA HIV1/O/2: High >1.46, and ABS QUAL: Repeatedly Reactive
2) Then they reflected with the same sample to a Supplemental test (bio-Rad Multispot): HIV 1- Non-Reactive, HIV-2 Non-Reactive
3) The recomendation for me was to get an RNA test, but I couldnt get one and instead I got an ECLIA test at 13 weeks - NEGATIVE (different lab)
4) Then at 22 weeks I did another ICMA HIV1/O/2: Normal <1.00, and ABS QUAL: Non-Reactive (different Lab)
5) Then at 22 weeks I did a 4th generation HIV test: NEGATIVE (with same blood sample above, item 4)
6) Then at 32 weeks I did the RT-PCR-RNA Test: <20 copies/ml
7) Then at 35 weeks I purchased a Home Oraquick Oral swab Test: Non-Reactive
8) Then at 36 weeks I purchased a Home Oraquick Oral swab Test: Non-Reactive
9) Then at 37 weeks I purchased a Home Oraquick Oral swab Test: Non-Reactive
10) Then at 48 weeks I purchased a Home Oraquick Oral swab Test: Non-Reactive

I still nervous with the first positive test. Can I move on or I need more test? Can people test positive and then test negative? Does antibodies disappear? Thanks


Subscribe to RSS - Pooled RNA/Early Test