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Western Blot

Haven't had sex in 3 and a half yrs. How can I be positive.

I was tested twice. Once on September and again in October of 2015. Now four months later with no sexual contact. No needles or any at risked involvement by me. I was tested again and came up positive. My second results should be in by March the 1st. I'm confused. Was it a false positive I received and how often does that happen. The fact that June will make 4 yrs since I had sex. Could it actually take 3 and a half yrs for virus to show up?

Please help me

at dec 5 2015.today is 85 days.i tested 7 weeks Anti hiv1/2 P24 antigen test and western blot.they came back non reaction.again i tested 9 weeks Anti hiv1/2 P24 antigen test and 11 weeks(78days).they came back non reaction too.they are conclusive?our country's doctors said Anti hiv1/2 P24 antigen test result is conclusive at 6 months.now i feel worry for my hiv status.our country is not enough for hiv treatment too.i will test at 90 days Anti hiv1/2 P24 antigen test that result will be conclusive? please reply me pleaseee advice me tooo thanks you so much.

hiv westernblot test is reliable

is western blot test is conclusive after four months of exposure......i have hiv negative but i feel some symptoms last month like night sweats,pains at nighttime....so iam feeling very anxious due to this iam not feeling good in all my day to day activities please provide answer thanks in advance

Possible Causes of Indeterminate/Inconclusive Western Blot

Exactly 30 days post exposure (unprotected vaginal/oral sex) I had a rapid test done. It came with a faint line and then confirmed with a confirmatory test Western Blot. The results were indeterminate with no information about viral bands detection. The case manager who informed me about the test results explained that WB could come indeterminate if I was at the early stage of the infection and was still seroconverting or non-specific antibody reaction. She also said that if I had a cold virus at a time it could've caused the antibody test to show inconclusive results.
Are there any other reasons besides seroconversion, other autoimmune diseases and viral infections for Western Blot to show indeterminate?
I know that according to the CDC guidelines 3 month-mark is considered considerate, however, what if the patient is consistently indeterminate on the Western Blot? Does it mean I am negative or should I try other tests?
Thanks in advance for any answer.

Qualitative vs Quantitative PCR RNA

Could you please explain the difference between the Qualitative vs Quantitative PCR RNA HIV tests? I have heard that these tests are not FDA approved for diagnostic purposes as well as prone to false positives. However, I do not want to wait for 12 weeks for Western Blot antibody test, I want faster results. Should I choose Qualitative or Quantitative PCR RNA?

inconclusive Western Blot Test Result

I have tested reactive on a rapid test and then indeterminate on Western Blot test. This was exactly a month after my exposure. The STD clinic told me to return in 12 weeks to repeat the Western Blot test to confirm my HIV status.
I would like to start with my risk assessment:
Unprotected vaginal/oral sex with a male partner, lost my virginity to him. Partner tested negative right after being informed about my inconclusive test results. I believe I was not exposed to non-sexual ways to contract HIV such as needle sharing, blood transfusions, getting tattoos or piercings.
My questions are:
1. If I test indeterminate for the 2nd time would this be conclusive? Should I take PCR RNA test then?
2. Could common cold virus throw off Western Blot test results?
3. Could having HPV throw off Western Blot results?
Thanks in advance for any input.

Borderline ELISA - ONLY Female with Female sexual relationships

Dear reader

I recently took a blood test for a general check up and my HIV test results came back as borderline .

- I am 22 / Female
- Never had a sexual relationship with a man
- Never used toys ( dildos, etc )
- Never injected drugs or shared needles ( i smoke marijuana on daily basis but nothing else, not even cigarettes)
- I had unprotected Oral sex with 2 girls . One of them 1 year ago and the other one 3 months ago (both claimed to be healthy)
- I had a Flu shot 3 months ago
- Around 2 months ago i had Herpes on my lip for the first time but applied antibiotics on it so it went away quickly ( i guess i got that from a friend who i shared a Marijuana role with and after i saw she had the herpes on her lip )
- I have a tattoo and its 1 year old, it was done in a hygienic manner
- Currently i was diagnosed with having a urinary infection, but i don't have the symptoms so my Dr told me to retake the urine test
- My White blood cells count is 8200
- The only sickness i had experienced in the past year was a tommy flu ( last week and a lot of people in my university have it now )

since i didn't expect to have HIV at all the borderline result really terrified me and now i'm waiting for my PCR test results.
- What causes a borderline test results / what does it mean /?
- Given my condition, how reliable my test results are going to be please ?
- on the other hand this situation has caused me a lot of stress and all of this is happening while i am on my PMS, i can't sleep properly or eat properly. my heart rate average in the past 5 days is 98 while usually its on 65-70 . Could you maybe suggest a way that can help me reduce my anxiety please ?
- Can any of the factors mentioned above ( Flu shot, Herpes, Marijuana, alcohol, urinary infection) manipulate my test results both PCR and ELISA 3rd generation ?

- Which test is the most accurate one for detecting HIV after all ( amongst all the testing methods existing) ? as my research shows they all can be wrong for so many reasons ! How can i finally know if i have it or not ?

Thank you for your time .

PEP Query

Hello. I had an exposure on 18th April 2015 (receptive oral sex with blood in mouth). Next morning, I went to the local HIV clinic (10 hours after exposure) and was prescribed PEP for a duration of 28 days. I took the medication religiously and completed the 28 days prescription. After one month, I had an HIV test and it came back as negative. I re-tested after 3 months and it again came back as negative. I went back to the clinic yesterday (after nearly 8 months) and performed a rapid test. Again the result was negative. However, the doctor has requested for a western blot test for confirmation purposes as I was on PEP. He said that this test is normally done to ensure PEP has worked. I am now very worried. All my tests came back as negative. What if the western blot comes back as positive. I am very stressed at the moment and your advice will be highly appreciated.

Kind regards

Multiple negative test conlusive?

Hello, I had 1 protected enclosure about 16 weeks ago, I tested PCR RNA negative at 2 weeks, then negative 4gen combo (antigen and antibody) at 28 days and again negative at 13 weeks. Then I tested negative rapid blod test at 14 weeks. Are these negative results conclusive?

Is there any change I can get positive after? Do I need to test at 6 months? Can antibodies be detected at a further point or with just the antigen and RNA is fine to rule out a a possbility of infection? I want to start my monogany sex life again with confidence!

Queries ? Repost ?

Last test was take at 179th day ! Hiv1/2 ag/ab ( Eclia ) Non reactive!

Got tested at 23rd 28th 42nd 93rd 122nd 179th day !
All were hiv 4th generation tests !
One PCR rna test ( limit was 66.6ml copies ) All came out negative!
One NAT test ( viral screening hiv1/2 hep b hep c ( Roche Cobas taqman )

1) are this conclusive ?

2) any further tests needed ? At 9months or 12months ?
3) do I need to test by western blot ?
4) any possible of all tests being false negative ? 5) symptoms ? Improper stools ? Minor sore throat ? Bad feeling about being infected !
6) diff between NAT and id NAT tests?


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