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Western Blot

western blot after 10 weeks

sir i had a protected sex with lady prostitute . during sex she has used coconut oil as a lubricant .I was not getting why she is doing so after sex i have checked condom it was not broken . after some weeks molluscum contagiousm devloped in my genital area . i visited skin specilist he suggested to test for HIV 1 & HIV 2 also vdrl after 6 weeks of possible exposure . then for confirmation i did western blot test that test also come negative after 10 weeks
1) Is western blot is is conclusive after 10 weeks
2) even though there is no condom broken am i risk of getting HIV ?
3) can use of coconut oil weak the condom that can pass HIV virus .

molluscum contagisum

sir i had unprotected sex with a familier lady . after some time i noticed some bumps on my genitals i visited to skin specilist . he adviced to take vdrl & hiv 1 & hiv 2 test .I took both test both r negative . i took this test after 3.5 months after possible exposure . i have confirmed this test after 4 moths with western blot technique .
i dont have any other previous disorder like hep c , i am not receiving any kind of cancer treatement . i feel that i am completaly healthy no any kind of hiv symptom yet present .
I hear that molluscum contagisum is due to weak immunity system like AIDS . i am 26 year completaly healthy looking man . i am scared due to above sentence . i live in india . is there differance in window period of hiv each country . please tell me is there any need to worry more . i asked doctor but he said test again after 6 months but i read on internet mostly that window period of hiv is not more that 3 months . i am completly confused scared plz plz plz guid me that i can take charge of my healthy life.

western blot test should i stop worry

sir i had unprotected sex with a familier lady . after 3.5 months i cought some bumps so i visited a skin specilist . he diagnosed moluscum i suggested to test for hiv1/ hiv 2 also vdrl . i have did that test both result are negative . i have asked doctor should i stop worry he said test again after 6 months . but due to fear i have decided to go for western blot i did this test after 4 months and result again came nagative ........ now please tell me should i stop worry .... i read that molluscum is rare in healthy adults .. came only those who have weak immunity system due to immunideficienacy disease like hiv . i again came in fear . i think that i am heathy ... and only 10 moluscum was there in genital area .. doctor removed this with surgery after one month moluscum came back to my genital area ....... so i tested western blot after 4 months of possible exposure & that was negative ...so please guid me .. now is there any need to worry because of this molluscum ... i dont have any other symptoms of hiv .... please guid

hiv ? final query ? can i move on ??

hola :)
male -26

i had an exposure to Local sex worker in may 2015 in greece as tourist

post exposure i had flu kinda symptoms 2-3 weeks lasting for 1 month

with great courage i got tested

Hiv 4th generation test at 23rd (3weeks -Eclia ) 28th (4weeks -Eclia) 42nd (6 weeks -Eclia) 58th (8weeks -Eclia) 89th (13weeks - Elfa ) 93rd (13weeks -Eclia ) 103rd(14 -elfa weeks ) 105th (14th weeks - Cmia ) 122nd (17weeks - ELFA ) 179th (25weeks -Eclia ) 224th ( 32weeks - ELfa )and last test was 304th day ( 43 weeks almost 10 months-Eclia )

One hiv 1 pcr rna at 103rd day ( limit was 66ml copies )

one ID NAT for Hiv 1/2 hep b hep c at 124th day ( Viral screening )

one 3rd generation test at 66th day

now other stds

Took syphills test at 23rd 42nd 58th 93rd 179th day - all non reactive

Took herpes 1/2 IgM IgG non reactive at 42nd day @ 93rd day IgM coming out as equivocal and IgG was non reactive @103/104 ( two different lab ) - both IgG IgM was no. Reactive again at 179th day non reactive

Took hepatitis b antigen @ 58th 93rd 179th day non reactive
Took Anti hcv at 93rd and 179th day non reactive
Took chlyamdia IgA IgM IgG -negative Anti HCV - 93rd day

i smoke 3 ciggies a day drink once a while and smoke marijuna often ...
i am lil obese (over weight ) bmi being 29

i still have and keep getting some symptoms which seems to be relevant to hiv ?

green stools ? loose stools ? sore throat ? itching ? rashes ? and many more

1) are my tests conclsuive ?

2) factors like smoking drinking or being over weight cause false negative ?

3) i just got married and i am scared of infecting my wife

4 ) being vitamin d and b12 deficiency ? Effects on tests ?

5) Can hiv 1 pcr rna qualitative detect all subtypes/strains ( even some rare like e / crf /
group 0 ) of hiv 1 at 14.5 weeks?

6) can ID NAT ( viral screening for Rna/dna for hiv 1/2 detects all subtypes /strains ( even some rare crfs groups strains !at 124 day or 17.9 weeks?

7) What is diff between Nat or Id NAT ? Is id NAT accurate?

8) do I need to test by western blot or any more test any other method ?

9) piece of guidance and help ?

Inconclusive Results, ARS, Braces


I recently was tested for HIV and when my results came in I was called into the testing clinic. These were the results:

HIV 1/2 Ag/Ab Combo Screen: Reactive
HIV 1 p24 Antigen Screen: Non-reactive
HIV 1 Western Blot: Negative
HIV Final Interpretation: No HIV p24 antigen detected; inconclusive for HIV1/HIV2 antibodies

I have had two sexual partners in my life. With one we only had oral sex. With the second more recent one we had sex twice and used a condom both times (10 weeks prior to the first test) and the person that I had sex with said that he had been tested and was HIV free prior to sex. I am gay and was bottoming. Based on my history I questioned the result and immediately went for point of care rapid testing. The test came back negative and the doctor said that he questioned the initial result, thinking that there may be a non-HIV antibody in my blood triggering a false positive. However, he told me to abstain and sent off my blood for additional testing. I have about a week to go before I hear back. I have a few questions:

1. I have braces and I kissed someone deeply three weeks before the first test. Could I have contracted HIV in this way?
2. I have had cold/flu symptoms and swollen glands on and off for the past 3 or 4 weeks. Could these be symptoms of ARS? The symptoms seemed to be resolving and then worsened with the stress from the news of the first test.
3. What is the HIV 1/2 Ag/Ab Combo Screen? Is this the same as ELISA? How does it differ from the other tests that came out negative? What could lead to a false positive on this test?

Any info that you could provide me with would be great. Thanks.

Haven't had sex in 3 and a half yrs. How can I be positive.

I was tested twice. Once on September and again in October of 2015. Now four months later with no sexual contact. No needles or any at risked involvement by me. I was tested again and came up positive. My second results should be in by March the 1st. I'm confused. Was it a false positive I received and how often does that happen. The fact that June will make 4 yrs since I had sex. Could it actually take 3 and a half yrs for virus to show up?

Please help me

at dec 5 2015.today is 85 days.i tested 7 weeks Anti hiv1/2 P24 antigen test and western blot.they came back non reaction.again i tested 9 weeks Anti hiv1/2 P24 antigen test and 11 weeks(78days).they came back non reaction too.they are conclusive?our country's doctors said Anti hiv1/2 P24 antigen test result is conclusive at 6 months.now i feel worry for my hiv status.our country is not enough for hiv treatment too.i will test at 90 days Anti hiv1/2 P24 antigen test that result will be conclusive? please reply me pleaseee advice me tooo thanks you so much.

hiv westernblot test is reliable

is western blot test is conclusive after four months of exposure......i have hiv negative but i feel some symptoms last month like night sweats,pains at nighttime....so iam feeling very anxious due to this iam not feeling good in all my day to day activities please provide answer thanks in advance

Possible Causes of Indeterminate/Inconclusive Western Blot

Exactly 30 days post exposure (unprotected vaginal/oral sex) I had a rapid test done. It came with a faint line and then confirmed with a confirmatory test Western Blot. The results were indeterminate with no information about viral bands detection. The case manager who informed me about the test results explained that WB could come indeterminate if I was at the early stage of the infection and was still seroconverting or non-specific antibody reaction. She also said that if I had a cold virus at a time it could've caused the antibody test to show inconclusive results.
Are there any other reasons besides seroconversion, other autoimmune diseases and viral infections for Western Blot to show indeterminate?
I know that according to the CDC guidelines 3 month-mark is considered considerate, however, what if the patient is consistently indeterminate on the Western Blot? Does it mean I am negative or should I try other tests?
Thanks in advance for any answer.

Qualitative vs Quantitative PCR RNA

Could you please explain the difference between the Qualitative vs Quantitative PCR RNA HIV tests? I have heard that these tests are not FDA approved for diagnostic purposes as well as prone to false positives. However, I do not want to wait for 12 weeks for Western Blot antibody test, I want faster results. Should I choose Qualitative or Quantitative PCR RNA?


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