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Western Blot

Borderline ELISA - ONLY Female with Female sexual relationships

Dear reader

I recently took a blood test for a general check up and my HIV test results came back as borderline .

- I am 22 / Female
- Never had a sexual relationship with a man
- Never used toys ( dildos, etc )
- Never injected drugs or shared needles ( i smoke marijuana on daily basis but nothing else, not even cigarettes)
- I had unprotected Oral sex with 2 girls . One of them 1 year ago and the other one 3 months ago (both claimed to be healthy)
- I had a Flu shot 3 months ago
- Around 2 months ago i had Herpes on my lip for the first time but applied antibiotics on it so it went away quickly ( i guess i got that from a friend who i shared a Marijuana role with and after i saw she had the herpes on her lip )
- I have a tattoo and its 1 year old, it was done in a hygienic manner
- Currently i was diagnosed with having a urinary infection, but i don't have the symptoms so my Dr told me to retake the urine test
- My White blood cells count is 8200
- The only sickness i had experienced in the past year was a tommy flu ( last week and a lot of people in my university have it now )

since i didn't expect to have HIV at all the borderline result really terrified me and now i'm waiting for my PCR test results.
- What causes a borderline test results / what does it mean /?
- Given my condition, how reliable my test results are going to be please ?
- on the other hand this situation has caused me a lot of stress and all of this is happening while i am on my PMS, i can't sleep properly or eat properly. my heart rate average in the past 5 days is 98 while usually its on 65-70 . Could you maybe suggest a way that can help me reduce my anxiety please ?
- Can any of the factors mentioned above ( Flu shot, Herpes, Marijuana, alcohol, urinary infection) manipulate my test results both PCR and ELISA 3rd generation ?

- Which test is the most accurate one for detecting HIV after all ( amongst all the testing methods existing) ? as my research shows they all can be wrong for so many reasons ! How can i finally know if i have it or not ?

Thank you for your time .

PEP Query

Hello. I had an exposure on 18th April 2015 (receptive oral sex with blood in mouth). Next morning, I went to the local HIV clinic (10 hours after exposure) and was prescribed PEP for a duration of 28 days. I took the medication religiously and completed the 28 days prescription. After one month, I had an HIV test and it came back as negative. I re-tested after 3 months and it again came back as negative. I went back to the clinic yesterday (after nearly 8 months) and performed a rapid test. Again the result was negative. However, the doctor has requested for a western blot test for confirmation purposes as I was on PEP. He said that this test is normally done to ensure PEP has worked. I am now very worried. All my tests came back as negative. What if the western blot comes back as positive. I am very stressed at the moment and your advice will be highly appreciated.

Kind regards

Multiple negative test conlusive?

Hello, I had 1 protected enclosure about 16 weeks ago, I tested PCR RNA negative at 2 weeks, then negative 4gen combo (antigen and antibody) at 28 days and again negative at 13 weeks. Then I tested negative rapid blod test at 14 weeks. Are these negative results conclusive?

Is there any change I can get positive after? Do I need to test at 6 months? Can antibodies be detected at a further point or with just the antigen and RNA is fine to rule out a a possbility of infection? I want to start my monogany sex life again with confidence!
Thanks!

Queries ? Repost ?

PRespost
Last test was take at 179th day ! Hiv1/2 ag/ab ( Eclia ) Non reactive!

Got tested at 23rd 28th 42nd 93rd 122nd 179th day !
All were hiv 4th generation tests !
One PCR rna test ( limit was 66.6ml copies ) All came out negative!
One NAT test ( viral screening hiv1/2 hep b hep c ( Roche Cobas taqman )

1) are this conclusive ?

2) any further tests needed ? At 9months or 12months ?
3) do I need to test by western blot ?
4) any possible of all tests being false negative ? 5) symptoms ? Improper stools ? Minor sore throat ? Bad feeling about being infected !
6) diff between NAT and id NAT tests?

? Queries ? Confused

PRespost Last test was take at 179th day ! Hiv1/2 ag/ab ( Eclia ) Non reactive!
Got tested at 23rd 28th 42nd 93rd 122nd 179th day !
All were hiv 4th generation tests !
One PCR rna test ( limit was 66.6ml copies ) All came out negative! One id NAT test - viral screening ( Roche Cobras taqman ) hiv1/2 hep b Hep c

1) are this conclusive ?

2) any further tests needed ? At 9months or 12months ?
3) do I need to test by western blot ?
4) any possible of all tests being false negative ?
5) symptoms ? Improper stools ? Minor sore throat ? Bad feeling about being infected !

can I do Western blot after tested negative on elisa?

after the unprotected sex I have done three test after getting severe all ARS like symptoms from 5th month...im still having symptoms which put Kevin doubt because I still have head bumps, generalized swollen lymph nodes on neck and one on back head too, weaknesses and pain on hand and leg..it exist after 7 month of exposure which started on 5th month..
I did test on 5 month and 5 days after exposure method immunochromatography (negative)
next test élisa 4 gen on exact 6 month (negative)
another test immunochromatography on 6 month
and 23 days which is negative too
so what you say about my status??
is this conclusive?
I have given blood for western blot test which will take 1 month for result to come..why so long? and is this test Is deciding test for hiv status??

equivocal eia and subsequent rna naat

Hi
Feeling very anxious and looking for some guidance please.
My boyfriend went for routine bloodwork 6 weeks ago incl an hiv test. His dr said his first test was inconclusive but the second test was fine so he has nothing to worry about. I asked my boyfriend what the deal was with his first test - he doesnt know. He showed me the test results - "eia equivocal. Ag (screen) very abnormal" . The western blot was not done as a secondary bc "low eia signals" dont allow for useful results. So an rna naat was done - no rna found - "these tesults are inconsistent with hiv infection" . His drs final conclusion was " nonreactive"
I'm wondering:
1. What does the very abnormal on the Ag screen mean?
2. Why wasn't a western blot done?
3. How conclusive is the overall result since the rna naat came back clean?
I am so overcome with anxiety I cant take it another day. Ive spent sbout 40 hours online trying to sort this out and it has just made me more anxious. I just need an interpretation bc trying to do it myself is killing me. Please help me.
Thank you

about hiv test

dear sir

i have unprotected sex with a commercial female after 9days i tested a westron blot test that was negetive but after17days i have fever and knees pain so i am worried about it please suggest me what to do I am in big stress please suggest me.

thanks

Oral sex + 6 and a half months test negative

Hello Guys,

Thank you for answering my questions in advance!
I have had an contact with a true positive person. I received fellatio, thats all i have done!
That happened on January 27... my last test is on august 17 i think (among many other tests..)
I have been tested well over 10 times, I have had 3th gen elisa's, 4th gen duo tests, westernblots, and one time RNA PCR test on 9 week after exposure.

I have had many symptoms(i will not go into much details) the remaining symptoms are:
Isometric Tremor (when i flex my muscle and try to hold it just shakes and cant remain steady), brittle nails, cold feed, eyefloaters and some weight loss + pain in the head when i look right with my eyes.

I am considering to get another test, but I am too scared to do this now... I have had so much hard time, every test i engaged i died inside waiting for the dreaded news... THANK GOD I remained negative, but even after hearing so many negative tests, I still cannot get back to normal :(

Can you please advise me how to proceed in this kind of situation? Should i go to another test and if so, what is my chance of testing positive after almost 7 months negative duo test, 9 week RNA test - undetectable + westernblot ( and many more tests).

THank you! I love you!

What does <1.00 mean ?

I went to a clinic where my friend works got blood work done for an hiv test acouple days later I got results back that said hiv-1/hiv-2 antibodies reflex confirmation western blot non reactive <1.00 cut off <1.00
I did this test post 35 days (5 week after exposure. Im very scared I didn't ask for a western blot does that mean I had a positive before and they had to confirm it?
Do you think it will change after because it is borderline <1.00 ? Do people usually get less than <1.00 ??

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