« Go Back

Western Blot

Possible Causes of Indeterminate/Inconclusive Western Blot

Exactly 30 days post exposure (unprotected vaginal/oral sex) I had a rapid test done. It came with a faint line and then confirmed with a confirmatory test Western Blot. The results were indeterminate with no information about viral bands detection. The case manager who informed me about the test results explained that WB could come indeterminate if I was at the early stage of the infection and was still seroconverting or non-specific antibody reaction. She also said that if I had a cold virus at a time it could've caused the antibody test to show inconclusive results.
Are there any other reasons besides seroconversion, other autoimmune diseases and viral infections for Western Blot to show indeterminate?
I know that according to the CDC guidelines 3 month-mark is considered considerate, however, what if the patient is consistently indeterminate on the Western Blot? Does it mean I am negative or should I try other tests?
Thanks in advance for any answer.

Qualitative vs Quantitative PCR RNA

Could you please explain the difference between the Qualitative vs Quantitative PCR RNA HIV tests? I have heard that these tests are not FDA approved for diagnostic purposes as well as prone to false positives. However, I do not want to wait for 12 weeks for Western Blot antibody test, I want faster results. Should I choose Qualitative or Quantitative PCR RNA?

inconclusive Western Blot Test Result

Hi,
I have tested reactive on a rapid test and then indeterminate on Western Blot test. This was exactly a month after my exposure. The STD clinic told me to return in 12 weeks to repeat the Western Blot test to confirm my HIV status.
I would like to start with my risk assessment:
Unprotected vaginal/oral sex with a male partner, lost my virginity to him. Partner tested negative right after being informed about my inconclusive test results. I believe I was not exposed to non-sexual ways to contract HIV such as needle sharing, blood transfusions, getting tattoos or piercings.
My questions are:
1. If I test indeterminate for the 2nd time would this be conclusive? Should I take PCR RNA test then?
2. Could common cold virus throw off Western Blot test results?
3. Could having HPV throw off Western Blot results?
Thanks in advance for any input.

Borderline ELISA - ONLY Female with Female sexual relationships

Dear reader

I recently took a blood test for a general check up and my HIV test results came back as borderline .

- I am 22 / Female
- Never had a sexual relationship with a man
- Never used toys ( dildos, etc )
- Never injected drugs or shared needles ( i smoke marijuana on daily basis but nothing else, not even cigarettes)
- I had unprotected Oral sex with 2 girls . One of them 1 year ago and the other one 3 months ago (both claimed to be healthy)
- I had a Flu shot 3 months ago
- Around 2 months ago i had Herpes on my lip for the first time but applied antibiotics on it so it went away quickly ( i guess i got that from a friend who i shared a Marijuana role with and after i saw she had the herpes on her lip )
- I have a tattoo and its 1 year old, it was done in a hygienic manner
- Currently i was diagnosed with having a urinary infection, but i don't have the symptoms so my Dr told me to retake the urine test
- My White blood cells count is 8200
- The only sickness i had experienced in the past year was a tommy flu ( last week and a lot of people in my university have it now )

since i didn't expect to have HIV at all the borderline result really terrified me and now i'm waiting for my PCR test results.
- What causes a borderline test results / what does it mean /?
- Given my condition, how reliable my test results are going to be please ?
- on the other hand this situation has caused me a lot of stress and all of this is happening while i am on my PMS, i can't sleep properly or eat properly. my heart rate average in the past 5 days is 98 while usually its on 65-70 . Could you maybe suggest a way that can help me reduce my anxiety please ?
- Can any of the factors mentioned above ( Flu shot, Herpes, Marijuana, alcohol, urinary infection) manipulate my test results both PCR and ELISA 3rd generation ?

- Which test is the most accurate one for detecting HIV after all ( amongst all the testing methods existing) ? as my research shows they all can be wrong for so many reasons ! How can i finally know if i have it or not ?

Thank you for your time .

PEP Query

Hello. I had an exposure on 18th April 2015 (receptive oral sex with blood in mouth). Next morning, I went to the local HIV clinic (10 hours after exposure) and was prescribed PEP for a duration of 28 days. I took the medication religiously and completed the 28 days prescription. After one month, I had an HIV test and it came back as negative. I re-tested after 3 months and it again came back as negative. I went back to the clinic yesterday (after nearly 8 months) and performed a rapid test. Again the result was negative. However, the doctor has requested for a western blot test for confirmation purposes as I was on PEP. He said that this test is normally done to ensure PEP has worked. I am now very worried. All my tests came back as negative. What if the western blot comes back as positive. I am very stressed at the moment and your advice will be highly appreciated.

Kind regards

Multiple negative test conlusive?

Hello, I had 1 protected enclosure about 16 weeks ago, I tested PCR RNA negative at 2 weeks, then negative 4gen combo (antigen and antibody) at 28 days and again negative at 13 weeks. Then I tested negative rapid blod test at 14 weeks. Are these negative results conclusive?

Is there any change I can get positive after? Do I need to test at 6 months? Can antibodies be detected at a further point or with just the antigen and RNA is fine to rule out a a possbility of infection? I want to start my monogany sex life again with confidence!
Thanks!

Queries ? Repost ?

PRespost
Last test was take at 179th day ! Hiv1/2 ag/ab ( Eclia ) Non reactive!

Got tested at 23rd 28th 42nd 93rd 122nd 179th day !
All were hiv 4th generation tests !
One PCR rna test ( limit was 66.6ml copies ) All came out negative!
One NAT test ( viral screening hiv1/2 hep b hep c ( Roche Cobas taqman )

1) are this conclusive ?

2) any further tests needed ? At 9months or 12months ?
3) do I need to test by western blot ?
4) any possible of all tests being false negative ? 5) symptoms ? Improper stools ? Minor sore throat ? Bad feeling about being infected !
6) diff between NAT and id NAT tests?

? Queries ? Confused

PRespost Last test was take at 179th day ! Hiv1/2 ag/ab ( Eclia ) Non reactive!
Got tested at 23rd 28th 42nd 93rd 122nd 179th day !
All were hiv 4th generation tests !
One PCR rna test ( limit was 66.6ml copies ) All came out negative! One id NAT test - viral screening ( Roche Cobras taqman ) hiv1/2 hep b Hep c

1) are this conclusive ?

2) any further tests needed ? At 9months or 12months ?
3) do I need to test by western blot ?
4) any possible of all tests being false negative ?
5) symptoms ? Improper stools ? Minor sore throat ? Bad feeling about being infected !

can I do Western blot after tested negative on elisa?

after the unprotected sex I have done three test after getting severe all ARS like symptoms from 5th month...im still having symptoms which put Kevin doubt because I still have head bumps, generalized swollen lymph nodes on neck and one on back head too, weaknesses and pain on hand and leg..it exist after 7 month of exposure which started on 5th month..
I did test on 5 month and 5 days after exposure method immunochromatography (negative)
next test élisa 4 gen on exact 6 month (negative)
another test immunochromatography on 6 month
and 23 days which is negative too
so what you say about my status??
is this conclusive?
I have given blood for western blot test which will take 1 month for result to come..why so long? and is this test Is deciding test for hiv status??

equivocal eia and subsequent rna naat

Hi
Feeling very anxious and looking for some guidance please.
My boyfriend went for routine bloodwork 6 weeks ago incl an hiv test. His dr said his first test was inconclusive but the second test was fine so he has nothing to worry about. I asked my boyfriend what the deal was with his first test - he doesnt know. He showed me the test results - "eia equivocal. Ag (screen) very abnormal" . The western blot was not done as a secondary bc "low eia signals" dont allow for useful results. So an rna naat was done - no rna found - "these tesults are inconsistent with hiv infection" . His drs final conclusion was " nonreactive"
I'm wondering:
1. What does the very abnormal on the Ag screen mean?
2. Why wasn't a western blot done?
3. How conclusive is the overall result since the rna naat came back clean?
I am so overcome with anxiety I cant take it another day. Ive spent sbout 40 hours online trying to sort this out and it has just made me more anxious. I just need an interpretation bc trying to do it myself is killing me. Please help me.
Thank you

Pages

Subscribe to RSS - Western Blot