« Go Back

Do I need a further Test?


I had a encounter of mutual masturbation, where I fingered the lady and then masturbated self with Vaginal Fluid on my fingers and that fluid may have came in contact with my Penis.

I had SERUM HIV(I & II)AG/AB( Combo) Test with Method : Chemiluminescent Immunoassay on day 15th and Day 29th and both the results are as under:

15th Day: 0.24 S/Co : Non Reactive
29th Day: 0.11 S/Co : Non Reactive

1. Can I Conclude that I am not infected on the basis of the Test results?
2. Is the above mentioned test 4th generation?
3. If I need to take the same test for a conclusive result, when should I take it again?

Thanks in advance for your knowledge sharing and support.



Thank you for your inquiry. From what we gather from the question, you were asking about the risk of acquiring HIV through mutual masturbation. From the information given, this scenario is determined to be No Risk (transmission of HIV is not possible in the given scenario).

The scenario mentioned above does not meet the three components of the Transmission Equation because the vaginal fluid is exposed to oxygen and would not have direct access to your bloodstream. HIV is inactivated very quickly once exposed to oxygen outside of the body, making the virus unable to cause disease in a new person (1). Also, non-insertive masturbation is an activity with no HIV risk because there is no way for the virus to enter the body.

In regards to your questions about testing, it is first important to note that since this scenario is determined to be no risk, an HIV test is not recommended. For your knowledge, I will still address each of your questions:

  1. HIV tests conducted during the window period (before 6 weeks post-exposure for a 4th Generation test) are not considered conclusive.
  2. The test you took is a 4th generation test. Any test that includes detection of both HIV antibodies (Ab) and HIV antigen (Ag) are 4th generation tests. Tests that only detect HIV Ab are typically known as 3rd generation tests.
  3. Most HIV specialists agree that a 4th generation test in considered conclusive after 6 weeks (2). Some guidelines and physicians recommend re-testing after 3 months for completely conclusive results.

Recommendation: No need for further HIV testing with the scenario provided, refer to a physician for other health related questions.


AIDS Vancouver Helpline/Online, Marie