Dear Volunteer of Aidsvancouver,
First of all thank you you guys so much for educating people. I am now facing with a very concern problem that I cannot stop thinking.
I already read the article that HIV virus cannot stay outside human, but the article also said that the optimum temperature for HIV virus is 37celcious.
I went to the country of hot weather which is approximately 37degree celcious when I check the weather from my phone. I went there to do the bycicle trial ( one kind of sport). Before I had a ride, i went to the toilet and I feel some fluid touched my vagina when I sat on the toilet seat (maybe blood), but at that time I didn’t think too much because I knew that people cannot get HIV infected by using public restroom. But after the toilet, I went on biking and had an accident on my vagina due to the unsmooth road and my vagina hit with the bicycle. After the biking I hurt my vagina when peeing and at the time I realized that before the bike my vagina touched with some fluid in the toilet seat before! I am now concern because the country I went had 37 degree celcious, so I worried that when the last person used the toilet and there fluid still on the surface and the virus will still alive due to the optimum temperature. And also I have an accident while biking the fluid can easily access to that wound on my vagina.
Due to the situation, do I need to concern on anything? Do I had risk with this situation?
And I want to know that if HIV virus gonna be inactive if it come out the human and face with oxygen but in hot country which approximately 37 celcious? Can we use the same rule with cold country?
Sorry for my poor English
Thank you for your inquiry. From what we gather from the question, you were asking about whether or not you might have acquired HIV after using a toilet seat that you believe had blood on it. You are also worried that your biking accident may have created an easy access for the HIV virus to enter your vagina. From the information given, this scenario is determined to be No Risk (Transmission of HIV is not possible in the given scenario).
No Risk: transmission of HIV is not possible with the given scenario, because no exchange of bodily fluids occurred. One cannot acquire the HIV virus after being in contact with urine or blood on a toilette seat. The blood on the toilette seat was exposed to the air (oxygen), and thus rendered the HIV virus inactive. Furthermore, the vagina wound (easy access) is also not a risk because there was no exchange of bodily fluids.
HIV is not transmitted through social contact such as hugging, kissing, shaking hands, sharing toilette or dishes. 
Further, to address your question about temperature, temperature does not discriminate against HIV, unless it is in scientific controlled altered environment for research purposes . Whether you are in a cold or hot environment, the HIV virus will not be transmitted as long as it is exposed to the air (whether it is hot or cold air).
Recommendation: No need for HIV test with the scenario provided, refer to a healthcare professional for other health related questions.
Regards, AIDS Vancouver Helpline/Online, Heidi