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pcr dna and p24combo test

Question: 

Hi
One of my friend participated in sex with a girl but the condom was broken in middle...he completed his sex after that he find it was broken...any way he tested with pcr dna qualitative test after 22 days from exposure the result is negative...and after 35 days from exposure tested with hiv duo p24 combo test .
the result is negative. After 60 days from exposure tested with same p24 combo test result is negative...pls anybody can give detailed information about this early tests...we saw in some websites the pcr dna test works 2 to 3 weeks...after 3 weeks or after a month or later the pcr dna test detect the virus or not ...it detect only in early stage like 2 to 3 weeks...or the pcr dna qualitative test detect virus after 3 months or not ..and also the antigen p24and hiv duo combo test also mentioned that after the seroconversion period the antigens are undetectable...
We are in confusion..the p24 combo test results was valid or not after 5 weeks and 8 weeks after exposure...
if test with pcr rna qualitative test is that usefull after 2 months from exposure
my friend dont have any symptoms during this 2 months...but he had sex with his wife and he used condom during the sex..but he kissed her with lips with sour lips..both of them have sour lips cause of summer heat..
is there any problem with lip sour contact with kissing...pls answer with full details...thanq u.

Answer: 

Hello and thanks for contacting AIDS Vancouver with your question.

It seems you are concerned that your friend might have contracted aids after a condom broke during sex although he has been tested 8 weeks after the exposure and results were negative. We are glad to help.

If a condom breaks during sex then the activity is regarded as unprotected sex. Unprotected sex is considered a High Risk activity. This means that studies have repeatedly associated this with HIV infection. However in your friend's case they have tested several times and at weeks after the exposure the results came back negative. That is encouraging. The tests that are carried out these days are quite advanced and have a high accuracy rates. Here is some information about testing.

Test Name
Method
Window Period
Conditions
Enzyme Immunoassay Antibody (EIA) 3rd Generation (ELISA) Blood test that looks for antibodies. Up to 95% of infections are detectable within 4-6 weeks post exposure. Most people develop detectable antibodies in 21-25 days. Most commonly available testing method. Conclusive at 3 months post exposure.
Test Name
Method
Window Period
Conditions
Rapid or “Point-of-Care” Blood or oral swab test that looks for antibodies. Up to 95% of infections are detectable within 4-6 weeks post exposure. Most people develop detectable antibodies in 21-25 days. The rapid test is a type of 3rd Generation test. Two forms available: finger prick blood sample or oral swab. Oral swab test is most common in the U.S. but due to false positives in Canada it is not approved and blood collection is more likely. Many places in the U.S. and abroad may charge a fee for rapid testing. Conclusive at 3 months post exposure.
Test Name
Method
Window Period
Conditions
4th Generation EIA Blood test that looks for antibodies AND p24 protein antigens. Commonly referred to as the "combination," "combo" or "DUO" test. P24 protein is detectable immediately after infection but only for the first few weeks. The antibody (ab) test has a window period of 4-12 weeks post exposure. This test is widely available in North America. Most HIV specialists consider this test to be conclusive at 6 weeks but official HIV testing guidelines still recommend re-testing at 12 weeks for conclusive results. Conclusive at 3 months post exposure.

The 4th generation or the combo test (p24) is regarded as the most accurate and some specialists accept that the results of this test six weeks after exposure are conclusive. At Aids Vancouver we recommend that people get tested 3 months (12 weeks) after the most recent exposure in order to have conclusive results. We do not determine the HIV status of an individual using symptoms. Testing is the only accurate way to determine your HIV status.

Kissing does not lead to HIV infection. When HIV comes into contact with saliva it is not transmittable. You can read more on this from the following website, How Do You Get Aids or from related postings on our Helpline Online forum.

If you have additional questions feel free to get in touch with us again.

Thanks for contacting AIDS Vancouver.

In health

Lawrence

AIDS Vancouver Online Helpline
helpline.aidsvancouver.org
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