I have done sexual activity with trans woman sex worker for the first time, Both used condom, I received anal from the trans woman but after reading on internet i got anxiety that the condom was broken or had tears and that is just like condom was not used! The meeting was not too long, was like 1 hour.
I have done PCR RNA and Ab tests at day 8, with other testes like Hep B,C and Syphilis. Thankfully all were negative. After that i read in the Internet that PCR RNA test is not accurate and I read that there is 4th gen test Ab/Ag that can be done after 28 days.
I have done the DUO test at the start of day 28. The result was negative.
I still have anxiety and it is irritating my overall health.
I would appreciate your kind response. Should I stay worried?
Thank you for your inquiry. From what we gather from the question, you were asking about the conclusiveness of your test results. The PCR RNA test has a window period of 2-3 weeks after exposure with a 99.6% accuracy, at day 8, the results of that test would not be considered conclusive. The DUO test is a 4th generation test that has a window period of 4-12 weeks, at day 28 after exposure, the test results would be considered conclusive with a 99.9% accuracy. 
In regards to using condoms for HIV prevention, when used correctly and consistently, condoms are highly effective in preventing HIV and other sexually transmitted infections.  Incorrect use of a condom can cause it to break, slip or leak during sex, compromising its effectiveness. Unprotected anal or vaginal sex is considered to be a high risk activity.
Please see the attached resource for the correct way to use a male condom: https://www.cdc.gov/condomeffectiveness/male-condom-use.html
Regards, AIDS Vancouver Helpline/Online, Ashley
- CATIE. HIV Testing Technologies. [Internet]. catie.ca [cited September 19, 2018]. Available from: https://www.catie.ca/index.php?q=en/fact-sheets/testing/hiv-testing-technologies.
- WHO. Condoms for HIV prevention. [Internet] who.int [cited September 19, 2018]. Available from: http://www.who.int/hiv/topics/condoms/en/