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Kindly help

Question: 

Dear,
On 11 Oct I tested for HIV at one lab, it was negative result. I have fear that they might have used a used needle. I am not sure.
So I want to retest. when should I retest. On 11 Jan, three months will complete.
Let me know if I should go for ELISA method OR CMIA method which can assure me of result on or after 11 Jan.
I am in stress and I would not be taking any test afterwards. I will rely on this final test which i have to do.
However it was general lab so I am not sure whether they used new /used needle. and also not sure that even if it is used needle, that needle contain HIV or not.
Can I relay on result of this coming test. suggest test method Elisa/CMIA to confirm. I am going to marry in Month of JAN last week. Need to confirm it before that anyhow.
Kindly suggest and help.

Answer: 

Hi There,
Thank you for using the AIDS Vancouver Helpline as your source for HIV/AIDS related information. I hope that the following information will ease your stress:
Third generation EIA (also known as ELISA) tests are the most commonly available testing methods within Canada with an accuracy of 99.8% with 95% of infections detected between 4-6 weeks and can be considered conclusive at 12 weeks.
The CMIA test is also known as the 4th generation EIA test. They look for the Antigen P24 protein (Ag) as well as the antibodies (Ab). The Ab portion of the test can detect 95% of infections at 6 weeks while the P24 is detectable immediately after infection (3-4 days post exposure) for the first 2-4 weeks. After 6 weeks this test is considered to be 99.9% accurate and conclusive by many specialists. That said, official guidelines still recommend re-testing at 12 weeks for completely conclusive results. The only advantage to taking this test is that it is able to detect infections sooner after an exposure that a 3rd Generation ELISA but for a 3 month test, either test is fine.
Both of these tests are very accurate after the 3 month period and you should have confidence in the results from either. Given your previous concern with a used needle during testing, I would recommend that you request the nurse open the needle to be used in front of you so that you know that it is sterile.
Please let us know if we can provide any more information. Or if you would like to call and speak to someone at the Helpline, you can do so anonymously and confidentially by calling 640.696.4666, Monday to Friday between 9am-4pm.
 
Cheers,
Cameron
AIDS Vancouver Helpline Volunteer
604.696.4666, Monday-Friday 9am-4pm PST
helpline@aidsvancouver.org