whitedoor2000
So I went to a clinic and the guy put my blood on the sample pad and said you have to wait one minute before you add the chase buffer. But he ended up messing around with the clock (as I timed it in my head) and it seemed like he waited more like 2 minutes before adding the chase buffer. I saw a control line, and my result was non-reactive (this is years after a possible exposure). Would waiting 2 minutes to add a chase buffer instead of 1 make a difference? 

Thank you!
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helpline-volunteer
Hi there,

Thank you for your inquiry. 

As you mentioned, the Alere Determine test involves a fingerstick sample being applied to a sample pad, followed by a drop of chase buffer. The chase buffer should be applied 1 minute after the blood has been transferred to ensure it doesn't overflow the sample pad (1). After reviewing the product insert, I don't see any indication that the addition of the chase buffer is time sensitive. Therefore, whether or not it was applied at the 1 minute mark or closer to 2 should be irrelevant. 

Additionally, if there were a problem with the test, the control line would not appear (meaning the test is invalid and needs to be repeated) (2). You mentioned you saw the control line appear, therefore the test should be considered valid in this case. 

AIDS Vancouver Helpline/Online, Ashley 

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Additional Resources:
Alere Determine Test Information (1)
Alere Determine Package Insert (2)
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