Anonymous
hello sir good day to you hope every thing is going good..
after all the tests i had done which is:
-Antibodies test .3 days later onit was negative (I know it was in vain).
-Antibodies test (eclia).10 days after exposure .it was negative (0.07).
_Hiv1-2 abs+p24 AG (CMIA).21 days after exposure . non reactive (0.08 s/co).
-Hiv1-2 PCR RNA..21 days after exposure . not detected
i decided to take another test today which is day no 69 i went and idid the test and please help me answering the following questions:
-on the report it was written ECLIA TEST so is it the same of ELISA TEST some thing else ?
– what is this test looking for exactlly antibodies or antigen or both togethore?
-the result was 0.25 negative does the increament on the number mean anything ?cause it was 0.07 on the first week.
and 0.08 on 3rd week.
thanks again and waiting for your kind response
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Anonymous
Hi there,

Thanks for using the AIDS Vancouver Helpline as a source for HIV related information.

Firstly, I would like to say that AIDS Vancouver abides by the World Health Organization guidelines, which state that HIV test results are conclusive when testing is conducted 84 days (or 12 weeks) post-exposure. At day 69, your ECLIA test (or 4th Generation test) is a very good indicator of a negative HIV status, but only at 84 days is your test result considered conclusive. That being said, we at AIDS Vancouver have never seen a negative result turn into a positive one after retesting, so it is extremely likely that you are, in fact, HIV negative.

Now let's get into your test results. Index values (e.g. 0.25, 0.07) are of no importance to us and are for lab technology only. Index values go up and down on a regular basis, just as your blood pressure does. Instead, it is your results that matter. All your tests have come back negative/non-reactive, which is an excellent indication of an HIV negative status.

The ECLIA test and ELISA test are different. The ECLIA test is the 4th Generation test. Also called the "Combo" or "DUO test", this test looks for antibodies and P24 protein antigens. As for your results: some HIV specialists consider the 4th Generation test to be conclusive at 6 weeks post-exposure, but here at AIDS Vancouver we still stand by the WHO guidelines of 12 weeks. Therefore, your negative ECLIA test results are an extremely good indicator of an HIV negative status, but we still recommend retesting at the 84 day mark.

The ELISA test, on the other hand, is the 3rd Generation test. This test looks for antibodies -- not for both antibodies and P24 protein antigens.

I trust I have addressed your concerns. Should you have any further questions, please feel free to contact us back.

In good health,

Tiina

AIDS Vancouver Volunteer
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