Anonymous
BAck in July 2014 (July 10) I have unprotected sex. August 06 2014 I have an overall health check including VIH test (p24 HIV 1/2). The results came back positive. Then on August 14, 2014 I went I took an ECLIA 4th GEneration test and came back negative. With the same sample, they tested again and it was negative. Should I be worry? IS this conclusive? Please help.
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Anonymous
Hi there, thank you for contacting the AIDS Vancouver Helpline for HIV and AIDS related information.

There is no need to worry too much! Your fourth generation tests should be a very good indication of you HIV status. The fourth generation test detects both antigens and antibodies in the blood and is therefore the test that is most likely to detect the presence of the virus in the body, especially at 30-40 days post exposure. With respect to the p24 HIV 1/2 test that you took, this test detects HIV antigens in the blood and is only an effective test if it is done within 28 days of your exposure. You took this test more than 28 days after your exposure and therefore it should not have been able to detect the virus to begin with! False positives do occur and occur much more frequently than false negatives, which are very rare. False positives occur primarily due to human error in the testing procedure, so it is quite possible that this was the case in your situation. The fourth generation tests are considered to be one of the best and most accurate tests to take.

All that being said, according to the World Health Organization, no HIV test is considered absolutely conclusive until 3 months post exposure. You may therefore want to consider retesting for HIV at 3 months (after October 10th in your case).

I hope this alleviates some of your anxiety, based on the tests that you had done it is very unlikely that you have HIV. Keep in mind also that the HIV virus is actually very difficult to transmit, even with unprotected sex. If you have any further questions regarding testing or transmission please feel free to contact us again or visit [Avert.org](http://www.avert.org/hiv-testing-frequently-asked-questions.htm) which has lots of reliable information.

In health,

Arne, AIDS Vancouver Helpline Volunteer
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