Thank you for your inquiry. From what we gather from the question, you were asking about the risk of acquiring HIV through using your hand, that was previously touching a nipple with potential exposure to breast milk, to masturbate. From the information given, this scenario is determined to be No Risk (transmission of HIV is not possible in the given scenario).
The scenario mentioned above does not meet the three components of the transmission equation. It does not satisfy the equation because any breast milk on your hand would be exposed to oxygen, rendering the HIV virus inactive (1). Further, masturbation is not an activity that provides risk for HIV transmission.
Recommendation: No need for HIV test with the scenario provided, refer to a healthcare professional for other health related questions.
AIDS Vancouver Helpline/Online, Marie
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1) Survival of HIV in the Environment