Anonymous
Dear

Had unprotected sex with a lady on 9th march 2016 (4-5 rounds on three occasions) letter when we tested the lady was positive with Determine. am worried, my first test with rapid test showed negative, in 21 days after last exposurer, Did PCR they said NO HIV DNA seen. in other laboratory after 56 days another laboratory results said my immune Virus-DNA quantitative less than 12iu/ml and this lies below cutoff point, but weak signal detected plus DNA, am infectious, when to another viral research centre at 58 days, they did rapid test (determine, stapak, unigol) all negative, cambo negative and try viral load, no HIV virus copies detected and at 76 days after last exposuser, determine still negative, which test should l believed in, am worried, becouse had even pharyngitis now but am being allergies to cold wherther and sit in a room with AC as an office.
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Anonymous
Hi there, and thanks a lot for contacting the AIDS Vancouver Helpline for your HIV/AIDS related health information. It seems you're concerned about the possibility of HIV transmitting during an encounter, and about the accuracy of some tests you've had since then. We're happy to answer your questions for you.

It's great you were proactive and went for testing, as unprotected sex is considered a high risk activity. This doesn't mean that a transmission occurred during your encounter, but that of the transmissions that have been observed, most have occurred due to activities such as these. To see the risk levels of various activities, we encourage you to check out our [risk assessment page. ](http://helpline.aidsvancouver.org/question/risk-assessment-chart). In the future, we encourage you to always use a condom for your encounters, as condom use substantially reduces the risk of transfer of HIV and other STIs. For more information on condom use, check out one of our favourite resources at [SmartSex](http://smartsexresource.com/topics/condoms).

Here is a bit more information on the tests you've had since your exposure:


Test Name Method Window Period Conditions
Rapid or “Point-of-Care” Blood or oral swab test that looks for antibodies. Up to 95% of infections are detectable within 4-6 weeks post exposure. Most people develop detectable antibodies in 21-25 days. The rapid test is a type of 3rd Generation test. Two forms available: finger prick blood sample or oral swab. Oral swab test is most common in the U.S. but due to false positives in Canada it is not approved and blood collection is more likely. Many places in the U.S. and abroad may charge a fee for rapid testing. Conclusive at 3 months post exposure
NAAT (PCR RNA & DNA) Nucleic-Acid Amplification Testing (NAAT) looks for the genetic material of HIV and tests for it with a Polymerase Chain Reaction (PCR) test. 2-3 weeks post exposure NAAT tests generally take two forms: DNA PCR and RNA PCR tests. DNA PCR NAAT tests are usually used for screening babies of HIV+ mothers. RNA PCR NAAT tests are often used to screen blood or organ donations. Both measure the viral load of a positive person’s blood. Conclusive at 3 months post exposure
You'll see in the charts that the tests you've had are not considered conclusive, meaning the results are not taken as accurate, until 3 months post exposure. Some of the tests you've had are really good indicators of your status, but you'll need to go for confirmatory testing at 3 months (or 90 days) post exposure.

As for the symptoms you are experiencing, we at AIDS Vancouver are not healthcare providers, so cannot comment on them. However, HIV infections are never diagnosed based on symptoms alone, simply because the symptoms of an HIV infection are quite common to many other common medical conditions. Testing is the only way to diagnose an HIV infection. If you're concerned about any symptoms you're experiencing, we'd encourage you to see a healthcare provider.


Thanks a lot for contacting the AIDS Vancouver Helpline with your question, we hope it has been answered fully.

Trevor

AIDS Vancouver Helpline/Online

helpline.aidsvancouver.org
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